mouse anti nf κb polyclonal antibody  (Abmart Inc)

Journal: Neural Regeneration Research

Article Title: Magnesium acetyltaurate prevents retinal damage and visual impairment in rats through suppression of NMDA-induced upregulation of NF-κB, p53 and AP-1 (c-Jun/c-Fos)

doi: 10.4103/1673-5374.310691

Figure Lengend Snippet: Flow chart of study design. n = Total number of rats per group. MgAT: Magnesium acetyltaurate; NF-κB: nuclear factor kappa-B; NMDA: N-methyl-D-aspartic acid; PBS: phosphate-buffered saline.

Article Snippet: Blots were then probed for overnight using 1:500–1:1000 dilutions of antibodies against β-actin (1:1000, mouse monoclonal beta-actin, #HC201-02, Transgen Biotech or #sc-47778, Santa Cruz Biotechnology), phospho-NF-κB p65 (1:500, mouse monoclonal anti-phospho-NF-κB p65, #sc-101752, Santa Cruz Biotechnology), NF-κB p65 (1:1000, mouse polyclonal anti-NF-κB p65, #PA1-30408, Thermo Scientific), phospho-p53 (1:500, mouse monoclonal anti-phospho-p53, #sc-135772, Santa Cruz Biotechnology), and p53 (1:1000, mouse monoclonal anti-p53, #sc-126, Santa Cruz Biotechnology) and AP-1 (1:500; rabbit polyclonal anti-AP-1, #ENT0244, Elabscience, Wuhan, Hubei Province, China).

Techniques:

Journal: Neural Regeneration Research

Article Title: Magnesium acetyltaurate prevents retinal damage and visual impairment in rats through suppression of NMDA-induced upregulation of NF-κB, p53 and AP-1 (c-Jun/c-Fos)

doi: 10.4103/1673-5374.310691

Figure Lengend Snippet: Primer sequence used for quantitative real-time polymerase chain reaction

Article Snippet: Blots were then probed for overnight using 1:500–1:1000 dilutions of antibodies against β-actin (1:1000, mouse monoclonal beta-actin, #HC201-02, Transgen Biotech or #sc-47778, Santa Cruz Biotechnology), phospho-NF-κB p65 (1:500, mouse monoclonal anti-phospho-NF-κB p65, #sc-101752, Santa Cruz Biotechnology), NF-κB p65 (1:1000, mouse polyclonal anti-NF-κB p65, #PA1-30408, Thermo Scientific), phospho-p53 (1:500, mouse monoclonal anti-phospho-p53, #sc-135772, Santa Cruz Biotechnology), and p53 (1:1000, mouse monoclonal anti-p53, #sc-126, Santa Cruz Biotechnology) and AP-1 (1:500; rabbit polyclonal anti-AP-1, #ENT0244, Elabscience, Wuhan, Hubei Province, China).

Techniques: Sequencing

Journal: Neural Regeneration Research

Article Title: Magnesium acetyltaurate prevents retinal damage and visual impairment in rats through suppression of NMDA-induced upregulation of NF-κB, p53 and AP-1 (c-Jun/c-Fos)

doi: 10.4103/1673-5374.310691

Figure Lengend Snippet: Effect of pre-treatment with MgAT on NMDA induced changes in NF-κB gene and protein expression as determined at 3, 6, 24 hours and 7 days post-treatment. (A) RT-PCR. The expression of NF-κB gene was normalized against GAPDH and β-actin. (B) Representative western blot bands (1: PBS, 2: NMDA, 3: 24MgAT + NMDA (pre-treatment with MgAT 24 hours before NMDA administration)). (C) Quantitative estimation of NF-κB protein expression as determined from densitometric analysis of western blot bands. The expression of NF-κB p65 protein was normalized against β-actin. Phosphorylation levels of NF-κB p65 were quantified relative to the total NF-κB p65 levels. PBS represents vehicle (control) group. There was a statistically significant difference between groups as determined by one-way analysis of variance, followed by post hoc Bonferroni analysis for multiple comparisons. Data represent three biological replicates with three technical replicates, n = 3 (mean ± SD), * P < 0.05, ** P < 0.01, *** P < 0.001, vs . corresponding time points in the PBS group; ## P < 0.01, ### P < 0.01, vs . corresponding time points in the NMDA group. MgAT: Magnesium acetyltaurate; NF-κB: nuclear factor-kappa B; NMDA: N-methyl-D-aspartate; PBS: phosphate buffered saline; phospho: phosphorylated; RT-PCR: reverse transcription-polymerase chain reaction.

Article Snippet: Blots were then probed for overnight using 1:500–1:1000 dilutions of antibodies against β-actin (1:1000, mouse monoclonal beta-actin, #HC201-02, Transgen Biotech or #sc-47778, Santa Cruz Biotechnology), phospho-NF-κB p65 (1:500, mouse monoclonal anti-phospho-NF-κB p65, #sc-101752, Santa Cruz Biotechnology), NF-κB p65 (1:1000, mouse polyclonal anti-NF-κB p65, #PA1-30408, Thermo Scientific), phospho-p53 (1:500, mouse monoclonal anti-phospho-p53, #sc-135772, Santa Cruz Biotechnology), and p53 (1:1000, mouse monoclonal anti-p53, #sc-126, Santa Cruz Biotechnology) and AP-1 (1:500; rabbit polyclonal anti-AP-1, #ENT0244, Elabscience, Wuhan, Hubei Province, China).

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot